ESTABLISHMENT OF A WELL-CHARACTERIZED SARS-COV-2 LENTIVIRAL PSEUDOVIRUS NEUTRALIZATION ASSAY USING 293T CELLS WITH STABLE EXPRESSION OF ACE2 AND TMPRSS2.

Establishment of a well-characterized SARS-CoV-2 lentiviral pseudovirus neutralization assay using 293T cells with stable expression of ACE2 and TMPRSS2.

Pseudoviruses are useful surrogates for highly pathogenic viruses because of their safety, genetic stability, and scalability for screening assays.Many different pseudovirus platforms exist, each with different advantages and limitations.Here we report our efforts to optimize and characterize an HIV-based lentiviral tess milne bikini pseudovirus as

read more


Isolation of serotype-specific antibodies against dengue virus non-structural protein 1 using phage display and application in a multiplexed serotyping assay.

The multidimensional nature of dengue virus (DENV) infections, which can be caused by four distinct serotypes of the virus, complicates the sensitivity of assays designed for the diagnosis jacques marie mage picabia of infection.Different viral markers can be optimally detected at different stages of infection.Of particular clinical importance is t

read more

Storage of Pitching Yeast in Brewery

This jacques marie mage picabia article deals with the issue of pitching yeast storage in brewery.The storage conditions before its repitching are a key factor for the quality of the fi nal product.A short overview of factors affecting viability and vitality of yeast during its storage is given, as well as the effect of the way and temperature of i

read more

Fishery induces sperm depletion and reduction in male reproductive potential for crab species under male-biased harvest strategy.

Sperm depletion in males can occur when polygynous species are intensively exploited under a male-biased management strategy.In fisheries involving crabs species, the effects of this type of management on the reproductive potential is far from being understood.This study tests whether male-biased management of the principal Chilean crab fishery is

read more